Application

Research Sub CategoryInflammatory DiseaseImmune ResponseAutoimmune disease

This new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-6 kit, #03-0089-01:
Improved options for sample prep and incubation
Lyophilization of the standard provides the kit at one storage condition
Overnight incubation can be used
Increased reagent volumes to accommodate automation
Validated to run on both SMCxPRO^® and Erenna™

Research CategoryInflammation & Immunology

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC™ Human Interleukin 6 (IL-6) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-6 in human serum and plasma.

Components

Assay BufferHuman IL-6 BeadsStandard DiluentHuman IL-6 Detection AntibodyHuman IL-6 Standard10X Wash BufferBuffer DElution Buffer B

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interleukin 6 (IL-6) is secreted by T cells and macrophages and acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Some of its functions include the stimulation of the immune response during infection, induction of fever in autoimmune, infectious and non-infectious diseases, the stimulation of acute phase reactions, differentiation of T cells, proliferation and differentiation of B-cells, the development of blood cells as well as the activation of osteoclasts and osteoporosis and the secretion of vascular endothelium growth factor (VEFG) to increase blood vessel growth and vascular permeability in inflammation. It is important for a short-term defense against infection, injury and inflammation however dysregulation of IL-6 results in disease.

IL-6 is important in the study of diseases such as diabetes, lupus, multiple myeloma, rheumatoid arthritis and some cancers such as prostate and pancreatic cancer. Conversely, deficiency of IL-6 can have an effect on immune activation and IgA antibodies while overexpression of IL-6 can result in an immunological imbalance leading to an autoimmune pathology.

Legal Information

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

Specifications

Minimum of 5 samples spiked with average recovery of 107% with a range of 90-114%.

Specificity

Although IL-6 was detectable in non-human primate (rhesus macaque and cynomolgus monkey) samples, the assay has not been validated for these species.